Hisat, Samtools, Bedtools
2.2.0, 1.10, 2.27.1
Rick Masonbrink
Dec. 12, 2022, 1:05 p.m.
Gene expression
RNA-seq of J2 PA3 nematodes (Hg-type 0) grown on a susceptible host (Williams 82 or EFX63) aligned with hisat2 --very-sensitive and according to strand information. Converted to bam and merged with Samtools, then converted to bigwig using bedtools genomcov and kentUtils.