Hisat, Samtools, Bedtools
2.2.0, 1.10, 2.27.1
Rick Masonbrink
Dec. 12, 2022, 1:38 p.m.
Gene expression
RNAseq from three reps of PA3 J2 gland cells aligned with Hisat2 --very-sensitive and according to strand information. Converted to bam and merged with Samtools, then converted to bigwig using Bedtools genomcov and kentUtils.