Ortholog based Manual Annotations
Orthofinder, custom bash scripts
Baum/Severin Labs
Oct. 29, 2018, midnight
Genes
This essentially works by taking the primary isoforms from each species, (B. xylophilus, D. destructor, G. rostochiensis, G. ellingtonae, G. pallida, M. hapla, M. incognita, M. floridensis, M. arenaria, M. javanica, and H. glycines) and running them through orthofinder to find orthologs. This assigns each gene with orthology to an orthogroup. For each H.glycines gene in an orthogroup, the genes for every other species were used to extract annotation information. This information was counted by using exact annotation matches for each gene. Thus for Hetgly.G000012040, a total of 146 gene annotations were found and sorted by most abundant annotation type -- 104-No-Annotation,64-Zinc-finger,-FLYWCH-type,37-MULE-transposase-domain,4-Zinc-finger,-FLYWCH-type- method:InterPro-accession:IPR018289,1-PAZ-domain,1-TSC-22-/-Dip-/-Bun. This information was added to the gff annotation of the genome using the Note= attribute.
reworkedfunctionalaugustus_sorted_gff0.gz